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Bicarbonate (CO2, HCO3-)

Clinical Biochemistry

Sample and container requirements

Serum Lithium Heparin Plasma (paediatrics only)

Minimum volume required

Special requirements

None

Patient preparation

None

Sample viability

Turnaround time

This assay is run throughout the day and night. The in-lab turnaround time is normally less than 24 hours.

Reference range

Neonate – 16 years: 19 – 28 mmol/L Adults: 22 – 29 mmol/L (Source: Pathology Harmony Recommendations)

Clinical guidance

Serum/Plasma bicarbonate measurements, combined with pH measurement are frequently used to diagnose and treat irregularities in acid-base imbalance. Please refer to LabTestsOnlineUK to learn more about bicarbonate. https://labtestsonline.org.uk/tests/bicarbonate

Notes

Artefactual decrease in bicarbonate can occur when samples are delayed in transit or if samples have been decapped for a prolonged period of time.

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This information was last updated on 19 August 2026