Pathology tests database

Sample and container requirements

Serum Lithium Heparin Plasma (paediatrics only)

Minimum volume required

Special requirements

Bilirubin is light-sensitive. Samples should be protected from exposure to sunlight and strong artificial light, as photodegradation can lead to falsely low bilirubin results.

Patient preparation

None

Sample viability

Turnaround time

This assay is run throughout the day and night. The in-lab turnaround time is normally less than 24 hours. The test can be ordered as an urgent request.

Reference range

<5 µmol/L (Birmingham Heartlands Hospital) < 9 µmol/L (Birmingham Queen Elizabeth Hospital)

Clinical guidance

Bilirubin is produced during the normal breakdown of red blood cells. It is processed by the liver, where it is converted into a water-soluble form (conjugated or direct bilirubin) before being excreted in bile and eliminated from the body. Bilirubin testing is used to investigate jaundice and assess liver function. Measuring total, direct (conjugated) and indirect (unconjugated) bilirubin can help distinguish between hepatocellular, cholestatic and haemolytic causes of jaundice. Raised conjugated (direct) bilirubin is commonly seen in liver disease, cholestasis and bile duct obstruction. Raised unconjugated (indirect) bilirubin is typically associated with haemolysis or inherited conditions such as Gilbert syndrome. In newborn babies, bilirubin measurement also helps identify conditions such as biliary atresia that require prompt diagnosis and treatment. Total bilirubin is the sum of direct (conjugated) and indirect (unconjugated) bilirubin. Please refer to LabTestsOnlineUK to learn more about bilirubin. https://labtestsonline.org.uk/tests/bilirubin

Notes