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        Bilirubin (Total)

        Clinical Biochemistry

        Sample and container requirements

        Serum Lithium Heparin Plasma (paediatrics only)

        Minimum volume required

        85 µL serum/plasma

        Special requirements

        Bilirubin is light-sensitive. Samples should be protected from exposure to sunlight and strong artificial light, as photodegradation can lead to falsely low bilirubin results.

        Patient preparation

        None

        Sample viability

        Turnaround time

        This assay is run throughout the day and night. The in-lab turnaround time is normally less than 24 hours. The test can be ordered as an urgent request.

        Reference range

        14 days – Adults: <21 µmol/L (Source: Pathology Harmony Recommendations)

        Clinical guidance

        Total bilirubin forms part of the liver function test profile. Please refer to LabTestsOnlineUK to learn more about bilirubin. https://labtestsonline.org.uk/tests/bilirubin Further information: NICE CG98 Neonatal Jaundice Guidelines: https://www.nice.org.uk/guidance/CG98

        Notes

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        This information was last updated on 19 August 2026