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        Cortisol (Saliva)

        Clinical Biochemistry

        Sample and container requirements

        Saliva (salivette)

        Minimum volume required

        500 µL saliva

        Special requirements

        None

        Patient preparation

        Individuals should avoid eating and brushing teeth for 30 minutes before collecting saliva samples to minimise the potential for blood contamination. Saliva specimens should be collected using a Sarstedt cortisol salivette. Saliva collected into a plain container by passive drool is also acceptable.

        Sample viability

        Turnaround time

        21 days

        Reference range

        Early morning range (7 – 9 a.m.): 3.2 – 22.8 nmol/L Late night range (11 p.m. – 12 a.m.): <3.2 nmol/L (Source: locally derived)

        Clinical guidance

        Please refer to LabTestsOnlineUK to learn more about cortisol. https://labtestsonline.org.uk/tests/cortisol-test

        Notes

        Salivettes for saliva collection can be ordered from the laboratory on the Queen Elizabeth Hospital site.

        Images to go here

        This information was last updated on 19 August 2026