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        Methotrexate

        Clinical Biochemistry

        Sample and container requirements

        Serum

        Minimum volume required

        Special requirements

        None

        Patient preparation

        For high dose IV regimes only. Low dose methotrexate - monitoring not required.

        Sample viability

        Turnaround time

        This assay is run throughout the day and night. The in-lab turnaround time is normally less than 24 hours.

        Reference range

        See individual protocols

        Clinical guidance

        Please refer to LabTestsOnlineUK to learn more about methotrexate. https://labtestsonline.org.uk/tests/methotrexate

        Notes

        Images to go here

        This information was last updated on 19 August 2026